Laboratory of Intermolecular Interactions
| CONTACTS |
MAIN RESEARCH
- Experimental interactomics of proteins and stable protein complexes
- Intermolecular interactions in membrane systems involving proteins
- Biosensor screening of drug prototypes and physiologically active substances: regulators of protein-protein interactions; inhibitors of enzymes and protein oligomerization
- Technologies of biosensor analysis and diagnostics of socially significant diseases
- Biosensor technologies: optical biosensors based on the effect of surface plasmon resonance (SPR), development of a methodology for the regeneration of optical chips and new principles of ligand immobilization.
- Research within the framework of state assignment 122030100168-2 “Development of experimental methods for studying the protein interactome, analysis of protein-protein and protein-ligand interactions, search for regulators of the functioning of target proteins and protein-protein interactions”
- Proteomic research within the framework of the scientific program of the biological satellite BION-M No. 2.
- Custom research projects on the topic "Biosensor SPR analysis of the interaction of secondary metabolites of marine fungi with estrogen receptors" (customer - PIBOC FEB RAS under grant RSF No. 25-73-20049, 2025-2027).
- Carrying out custom scientific researches (as services of “Human Proteome” Core Facilities) for commercial and academic customers (SPR analysis, molecular fishing, analysis of drug immunogenicity, screening of biologically active substances for their interaction with target proteins).
Kaluzhskiy L., Yablokov E., Varaksa T., Grudo A., Karputs A., Grabovec I., Mezentsev Y., Zelepuga E., Gnedenko O., Tolkanov D., Malyarenko T., Kolesnikova S., Kozhushnaya A., Vasileva E., Mishchenko N., Silchenko A., Avilov S., Rutckova T., Tarbeeva D., Kalinin V., Kicha A., Kozlovskaya E., Fedoreyev S., Ivanchina N., Dmitrenok P., Gilep A., Ivanov A. (2026) Diverse ligands for mycobacterial CYP124 identified from plant and marine compounds, Biochimie, 245, 40-54. DOI:10.1016/j.biochi.2026.02.019
Ershov P.V., Yablokov E.O., Kaluzhskiy L.A., Mezentsev Yu.V., Gnedenko O.V., Konstantinov M.A., Toropygin I.Yu., Ivanov A.S. (2025) Glycyrrhizic acid: novel potential protein targets, Biomeditsinskaya Khimiya, 71(4), 270-282. DOI:10.18097/PBMCR1595
Konstantinov M., Kaluzhskiy L., Yablokov E., Zhdanov D., Ivanov A., Toropygin I. (2025) Substrate Specificity and Peptide Motif Preferences of β-Lytic and L5 Proteases from Lysobacter spp. Revealed by LC–MS/MS Analysis, International Journal of Molecular Sciences, 26(17), 8603. DOI:10.3390/ijms26178603
Kaluzhskiy L., Yablokov E., Gnedenko O., Burkatovskii D., Maslov I., Bogorodskiy A., Ershov P., Tsybruk T., Zelepuga E., Rutckova T., Kozlovskaya E., Dmitrenok P., Gilep A., Borshchevskiy V., Strushkevich N., Ivanov A. (2024) The effect of membrane composition on the interaction between human CYP51 and its flavonoid inhibitor - luteolin 7,3′-disulfate, Biochimica et Biophysica Acta - Biomembranes, 1866(3), 184286. DOI:10.1016/j.bbamem.2024.184286
Tumilovich A., Yablokov E., Mezentsev Yu., Ershov P., Basina V., Gnedenko O., Kaluzhskiy L., Tsybruk T., Grabovec I., Kisel M., Shabunya P., Soloveva N., Vavilov N., Gilep A., Ivanov A. (2024) The Multienzyme Complex Nature of Dehydroepiandrosterone Sulfate Biosynthesis, International Journal of Molecular Sciences, 25(4), 2072. DOI:10.3390/ijms25042072
Gilep A., Varaksa T., Bukhdruker S., Kavaleuski A., Ryzhykau Y., Smolskaya S., Sushko T., Tsumoto K., Grabovec I., Kapranov I., Okhrimenko I., Marin E., Shevtsov M., Mishin A., Kovalev K., Kuklin A., Gordeliy V., Kaluzhskiy L., Gnedenko O., Yablokov E., Ivanov A., Borshchevskiy V., Strushkevich N. (2023) Structural insights into 3Fe–4S ferredoxins diversity in M. tuberculosis highlighted by a first redox complex with P450, Frontiers in Molecular Biosciences, 9, 1100032. DOI:10.3389/fmolb.2022.1100032
Ershov P.V., Yablokov E.O., Mezentsev Yu.V., Chuev G.N., Fedotova M.V., Kruchinin S.E., Ivanov A.S. (2022) SARS-COV-2 coronavirus papain-like protease PLpro as an antiviral drug target for inhibitors of active site and protein-protein interactions, Biophysics, 67, 902-912. DOI:10.1134/S0006350922060082
Kaluzhskiy L., Ershov P., Yablokov E., Shkel T., Grabovec I., Mezentsev Yu., Gnedenko O., Usanov S., Shabunya P., Fatykhava S., Popov A., Artyukov A., Styshova O., Gilep A., Strushkevich N., Ivanov A. (2021) Human Lanosterol 14-Alpha Demethylase (CYP51A1) Is a Putative Target for Natural Flavonoid Luteolin 7,3′-Disulfate, Molecules, 26(8), 2237. DOI:10.3390/molecules26082237
Ershov P., Kaluzhskiy L., Mezentsev Yu., Yablokov E., Gnedenko O., Ivanov A. (2021) Enzymes in the Cholesterol Synthesis Pathway: Interactomics in the Cancer Context, Biomedicines, 9(8), 895. DOI:10.3390/biomedicines9080895
Ershov P.V., Mezentsev Yu.V., Kopylov A.T., Yablokov E.O., Svirid A.V., Lushchyk A.Ya., Kaluzhskiy L.A., Gilep A.A., Usanov S.A., Medvedev A.E., Ivanov A.S. (2019) Affinity Isolation and Mass Spectrometry Identification of Prostacyclin Synthase (PTGIS) Subinteractome, Biology, 8(2), 49. DOI:10.3390/biology8020049