Laboratory of Intermolecular Interactions
| CONTACTS |
- Biosensor technologies: optical biosensors based on the effect of surface plasmon resonance (SPR), development of a methodology for the regeneration of optical chips and new principles of ligand immobilization.
- Research within the framework of state assignment 122030100168-2 “Development of experimental methods for studying the protein interactome, analysis of protein-protein and protein-ligand interactions, search for regulators of the functioning of target proteins and protein-protein interactions”
- Proteomic research within the framework of the scientific program of the biological satellite BION-M No. 2.
- Carrying out custom scientific research “Biosensory SPR analysis of interactions of low-molecular compounds from marine origin with target enzyme proteins” (as services of “Human Proteome” Core Facilities) Customer: G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS (according RSF grant 23-44-10009, 2023-2025).
- Carrying out custom scientific researches (as services of “Human Proteome” Core Facilities) for commercial and academic customers (SPR analysis, molecular fishing, analysis of drug immunogenicity, screening of biologically active substances for their interaction with target proteins).
Vasileva E.A., Berdyshev D.V., Mishchenko N.P., Gerasimenko A.V., Menchinskaya E.S., Pislyagin E.A., Chingizova E.A., Kaluzhskiy L.A., Dautov S.S., Fedoreyev S.A. (2024) Phanogracilins A–C, New Bibenzochromenones of Crinoid Phanogenia gracilis (Hartlaub, 1890), Biomolecules, 14(2), 151. DOI:10.3390/biom14020151
Kaluzhskiy L., Yablokov E., Gnedenko O., Burkatovskii D., Maslov I., Bogorodskiy A., Ershov P., Tsybruk T., Zelepuga E., Rutckova T., Kozlovskaya E., Dmitrenok P., Gilep A., Borshchevskiy V., Strushkevich N., Ivanov A. (2024) The effect of membrane composition on the interaction between human CYP51 and its flavonoid inhibitor - luteolin 7,3′-disulfate, Biochimica et Biophysica Acta - Biomembranes, 1866(3), 184286. DOI:10.1016/j.bbamem.2024.184286
Tumilovich A., Yablokov E., Mezentsev Yu., Ershov P., Basina V., Gnedenko O., Kaluzhskiy L., Tsybruk T., Grabovec I., Kisel M., Shabunya P., Soloveva N., Vavilov N., Gilep A., Ivanov A. (2024) The Multienzyme Complex Nature of Dehydroepiandrosterone Sulfate Biosynthesis, International Journal of Molecular Sciences, 25(4), 2072. DOI:10.3390/ijms25042072
Pislyagin E.A., Menchinskaya E.S., Gladkikh I.N., Kvetkina A.N., Sintsova O.V., Popkova D.V., Kozlovskiy S.A., Gorpenchenko T.Y., Likhatskaya G.N., Kaluzhskiy L.A., Ivanov A.S., Andreev Y.A., Kozlov S.A., Dmitrenok P.S., Aminin D.L., Leychenko E.V. (2023) Recombinant Analogs of Sea Anemone Kunitz-Type Peptides Influence P2X7 Receptor Activity in Neuro-2a Cells, Marine Drugs, 21(3), 192. DOI:10.3390/md21030192
Gilep A., Kuzikov A., Sushko T., Grabovec I., Masamrekh R., Sigolaeva L.V., Pergushov D.V., Schacher F.H., Strushkevich N., Shumyantseva V.V. (2022) Electrochemical characterization of mutant forms of rubredoxin B from Mycobacterium tuberculosis, Biochimica et Biophysica Acta - Proteins and Proteomics, 1870(1), 140734. DOI:10.1016/j.bbapap.2021.140734
Ershov P.V., Mezentsev Y.V., Ivanov A.S. (2022) Interfacial Peptides as Affinity Modulating Agents of Protein-Protein Interactions, Biomolecules, 12(1), 106. DOI:10.3390/biom12010106
Rodriguez-Antona C., Savieo J.L., Lauschke V.M., Sangkuhl K., Drögemöller B.I., Wang D., van Schaik R.H.N., Gilep A.A., Peter A.P., Boone E.C., Ramey B.E., Klein T.E., Whirl-Carrillo M., Pratt V.M., Gaedigk A. (2022) PharmVar GeneFocus: CYP3A5, Clinical Pharmacology & Therapeutics, 112(6), 1159-1171. DOI:10.1002/cpt.2563
Varaksa T., Bukhdruker S., Grabovec I., Marin E., Kavaleuski A., Gusach A., Kovalev K., Maslov I., Luginina A., Zabelskii D., Astashkin R., Shevtsov M., Smolskaya S., Kavaleuskaya A., Shabunya P., Baranovsky A., Dolgopalets V., Charnou Y., Savachka A., Litvinovskaya R., Hurski A., Shevchenko E., Rogachev A., Mishin A., Gordeliy V., Gabrielian A., Hurt D.E., Nikonenko B., Majorov K., Apt A., Rosenthal A., Gilep A., Borshchevskiy V., Strushkevich N. (2021) Metabolic Fate of Human Immunoactive Sterols in Mycobacterium tuberculosis, Journal of Molecular Biology, 433(4), 166763. DOI:10.1016/j.jmb.2020.166763
Yablokov E.O., Sushko T.A., Kaluzhskiy L.A., Kavaleuski A.A., Mezentsev Y.V., Ershov P.V., Gilep A.A., Ivanov А.S., Strushkevich N.V. (2021) Substrate-induced modulation of protein-protein interactions within human mitochondrial cytochrome P450-dependent system, Journal of Steroid Biochemistry and Molecular Biology, 208, 105793. DOI:10.1016/j.jsbmb.2020.105793
Sushko T., Kavaleuski A., Grabovec I., Kavaleuskaya A., Vakhrameev D., Bukhdruker S., Marin E., Kuzikov A., Masamrekh R., Shumyantseva V., Tsumoto K., Borshchevskiy V., Gilep A., Strushkevich N. (2021) A new twist of rubredoxin function in M. Tuberculosis, Bioorganic Chemistry, 109, 104721. DOI:10.1016/j.bioorg.2021.104721
Ershov P.V., Yablokov Е.O., Florinskaya A.V., Mezentsev Y.V., Kaluzhskiy L.А., Tumilovich A.M., Gilep А.А., Usanov S.A., Ivanov АS. (2019) SPR—Based study of affinity of cytochrome P450s / redox partners interactions modulated by steroidal substrates, Journal of Steroid Biochemistry and Molecular Biology, 187, 124-129. DOI:10.1016/j.jsbmb.2018.11.009
Meza-Menchaca T., Ramos-Ligonio A., López-Monteon A., Vidal Limón A., Kaluzhskiy L.A., V Shkel T., V Strushkevich N., Jiménez-García L.F., Agredano Moreno L.T., Gallegos-García V., Suárez-Medellín J., Trigos Á. (2019) Insights into Ergosterol Peroxide`s Trypanocidal Activity, Biomolecules, 9(9), 484. DOI:10.3390/biom9090484