Laboratory of Cell Biology
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Viktoria D. Novikova
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Elena I. Yarygina
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RESEARCH
The scope of the laboratory includes fundamental research aimed at the development of innovative cell-based technologies for regenerative medicine and oncology and the advancement of cell models for postgenomics research.
- Research embraces the immunomodulation activity of stem and progenitor cells including molecular mechanisms of their interaction with immune cells, as well as involvement in immune and inflammatory responses.
- Ischemic stroke, lymphedema, osteoarthrosis and other disease models are used to evaluate the efficacy and safety of cell therapy and to study its cellular and molecular mechanisms of action.
- Concepts and efficient methods of cell immunotherapy of oncological, autoimmune and chronic viral diseases are being developed. This involves targeted modification of cell populations, including genome editing.
- Cell heterogeneity of solid tumors, including colorectal cancer in the first place, is assessed. Special attention is paid to the disclosure of the molecular features of the population of cancer stem cells and to the tumor stromal cells immunology.
- Scaling-up methods for primary cell cultures, including hepatocyte cultures are being developed.
TEAM
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Members of the team are experts in a variety of cell culture techniques, including maintaining the 2D and 3D cultures of mesenchymal stem cells, neural stem/progenitor cells, induced pluripotent cells, keratinocytes, tumor cells, etc. Research is based on methods employing the intramural equipment: flow cytometric cell sorting (FACSAria III), flow cytometry with visualization (ImageStreamX Mark II), fluorescent microscopy using the innovative Leica THUNDER Imager with Computational Clearing system, “classical” confocal microscopy (Zeiss 200), cell culture dynamic observation and recording with IncuCyte Zoom, laser capture microdissection of histological slices (Arcturus XT), real-time PCR (Mx3005P Stratagene).
Saryglar R.Yu., Lupatov A.Yu., Yarygin K.N. (2023) Colorectal cancer and tumor stromal cells have different effects on the differentiation and maturation of dendritic cells in vitro, Bulletin of Experimental Biology and Medicine(Cell Technologies in Biology and Medicine), 174, 533-537. DOI:10.1007/s10517-023-05743-z
Kholodenko I.V., Kholodenko R.V., Yarygin K.N. (2023) The Crosstalk between Mesenchymal Stromal/Stem Cells and Hepatocytes in Homeostasis and under Stress, International Journal of Molecular Sciences, 24(20), 15212. DOI:10.3390/ijms242015212
Kholodenko I.V., Yarygin K.N. (2023) Hepatic Macrophages as Targets for the MSC-Based Cell Therapy in Non-Alcoholic Steatohepatitis, Biomedicines, 11(11), 3056. DOI:10.3390/biomedicines11113056
Cherkashova E.A., Namestnikova D.D., Gubskiy I.L., Revkova V.A., Sukhinich K.K., Melnikov P.A., Abakumov M.A., Savina G.D., Chekhonin V.P., Gubsky L.V., Yarygin K.N. (2023) Dynamic MRI of the Mesenchymal Stem Cells Distribution during Intravenous Transplantation in a Rat Model of Ischemic Stroke, Life, 13(2), 288. DOI:10.3390/life13020288
Vakhrushev I.V., Nezhurina E.K., Karalkin P.A., Tsvetkova A.V., Sergeeva N.S., Majouga A.G., Yarygin K.N. (2022) Heterotypic Multicellular Spheroids as Experimental and Preclinical Models of Sprouting Angiogenesis, Biology, 11(1), 18. DOI:10.3390/biology11010018
Gubskiy I.L., Namestnikova D.D., Revkova V.A., Cherkashova E.A., Sukhinich K.K., Beregov M.M., Melnikov P.A., Abakumov M.A., Chekhonin V.P., Gubsky L.V., Yarygin K.N. (2022) The Impact of Cerebral Perfusion on Mesenchymal Stem Cells Distribution after Intra-Arterial Transplantation: A Quantitative MR Study, Biomedicines, 10(2), 353. DOI:10.3390/biomedicines10020353
Kholodenko I.V., Gisina A.M., Manukyan G.V., Majouga A. (2022) Resistance of Human Liver Mesenchymal Stem Cells to FAS-Induced Cell Death, Current Issues in Molecular Biology, 44(8), 3428-3443. DOI:10.3390/cimb44080236
Kim Y.S., Potashnikova D.M., Gisina A.M., Kholodenko I.V., Kopylov A.T., Tikhonova O.V., Kurbatov L.K., Saidova A.A., Tvorogova A.V., Kholodenko R.V., Belousov P.V., Vorobjev I. A., Zgoda V.G., Yarygin K.N., Lupatov A.Yu. (2022) TRIM28 Is a Novel Regulator of CD133 Expression Associated with Cancer Stem Cell Phenotype, International Journal of Molecular Sciences, 23(17), 9874. DOI:10.3390/ijms23179874
Lupatov A.Yu., Yarygin K.N. (2022) Telomeres and Telomerase in the Control of Stem Cells, Biomedicines, 10(10), 2335. DOI:10.3390/biomedicines10102335
Kholodenko I.V., Kholodenko R.V., Majouga A.G., Yarygin K.N. (2022) Apoptotic MSCs and MSC-Derived Apoptotic Bodies as New Therapeutic Tools, Current Issues in Molecular Biology, 44(11), 5153-5172. DOI:10.3390/cimb44110351
Gisina A., Kholodenko I., Kim Y., Abakumov M., Lupatov A., Yarygin K. (2022) Glioma Stem Cells: Novel Data Obtained by Single-Cell Sequencing, International Journal of Molecular Sciences, 23(22), 14224. DOI:10.3390/ijms232214224
Namestnikova D.D., Gubskiy I.L., Revkova V.A., Sukhinich K.K., Melnikov P.A., Gabashvili A.N., Cherkashova E.A., Vishnevskiy D.A., Kurilo V.V., Burunova V.V., Semkina A.S., Abakumov M.A., Gubsky L.V., Chekhonin V.P., Ahlfors J.-E., Baklaushev V.P., Yarygin K.N. (2021) Intra-Arterial Stem Cell Transplantation in Experimental Stroke in Rats: Real-Time MR Visualization of Transplanted Cells Starting With Their First Pass Through the Brain With Regard to the Therapeutic Action, Frontiers in Neuroscience, 15, 641970. DOI:10.3389/fnins.2021.641970
Salikhova D., Bukharova T., Cherkashova E., Namestnikova D., Leonov G., Nikitina M., Gubskiy I., Akopyan G., Elchaninov A., Midiber K., Bulatenco N., Mokrousova V., Makarov A., Yarygin K., Chekhonin V., Mikhaleva L., Fatkhudinov T., Goldshtein D. (2021) Therapeutic Effects of hiPSC-Derived Glial and Neuronal Progenitor Cells-Conditioned Medium in Experimental Ischemic Stroke in Rats, International Journal of Molecular Sciences, 22(9), 4694. DOI:10.3390/ijms22094694
Gisina A., Novikova S., Kim Y., Sidorov D., Bykasov S., Volchenko N., Kaprin A., Zgoda V., Yarygin K., Lupatov A. (2021) CEACAM5 overexpression is a reliable characteristic of CD133-positive colorectal cancer stem cells, Cancer Biomarkers, 32(1), 85-98. DOI:10.3233/CBM-203187
Novikova S., Tikhonova O., Kurbatov L., Farafonova T., Vakhrushev I., Lupatov A., Yarygin K., Zgoda V. (2021) Omics Technologies to Decipher Regulatory Networks in Granulocytic Cell Differentiation, Biomolecules, 11(6), 907. DOI:10.3390/biom11060907
Lupatov A.Yu., Saryglar R.Y., Vtorushina V.V., Poltavtseva R.A., Bystrykh O.A., Chuprynin V.D., Krechetova L.V., Pavlovich S.V., Yarygin K.N., Sukhikh G.T. (2021) Mesenchymal Stromal Cells Isolated from Ectopic but Not Eutopic Endometrium Display Pronounced Immunomodulatory Activity In Vitro, Biomedicines, 9(10), 1286. DOI:10.3390/biomedicines9101286
Yarygin K.N., Namestnikova D.D., Sukhinich K.K., Gubskiy I.L., Majouga A.G., Kholodenko I.V. (2021) Cell Therapy of Stroke: Do the Intra-Arterially Transplanted Mesenchymal Stem Cells Cross the Blood–Brain Barrier?, Cells, 10(11), 2997. DOI:10.3390/cells10112997
Gubskiy I.L., Namestnikova D.D., Sukhinich K.K., Revkova V.A., Melnikov P.A., Gubsky L.V., Chekhonin V.P., Yarygin K.N. (2021) MRI-Based and Histologically Verified 3D Modeling of Spatial Distribution of Intra-Arterially Transplanted Cells in Rat Brain, Bulletin of Experimental Biology and Medicine(Cell Technologies in Biology and Medicine), 171(4), 517-522. DOI:10.1007/s10517-021-05263-8