Laboratory Of Bioelectrochemistry
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Polina I. Koroleva
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Alina V. Berezhnova
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RESEARCH
- Electrochemistry of biological objects (proteins, DNA, drugs, amino acids)
- Development of electrochemical methods for the analysis of bioaffinity interactions
- Analysis of enzyme-substrate interactions using electrochemical methods
DIRECTION OF INVESTIGATION
- Electrochemistry of biological objects (proteins, DNA, medicines, amino acids)
- Development of electrochemical methods for the analysis of bioaffinity interactions
- Analysis of enzyme-substrate interactions using electrochemical methods
- Electroanalysis of enzymes (cytochromes P450) for assessment of genetic polymorphisms and specifics of pharmacokinetics
- Electrochemical analysis of transfection
- Analysis of DNA degradation during apoptosis and action of restrictases and nucleases
- Electrochemical analysis of the catalytic activity of clinically important enzymes, such as cytochromes P450, asparaginase, proteases (trypsin, the main coronaviral protease Mpro), β-lactamases, restrictases, nucleases
Shumyantseva V.V., Koroleva P.I., Bulko T.V., Shkel T.V., Gilep A.A., Veselovsky A.V. (2023) Approaches for increasing the electrocatalitic efficiency of cytochrome P450 3A4, Bioelectrochemistry (Amsterdam, Netherlands), 149, 108277. DOI:10.1016/j.bioelechem.2022.108277
Shumyantseva V.V., Bulko T.V., Agafonova L.E., Pronina V.V., Kostryukova L.V. (2023) Comparative Analysis of the Interaction between the Antiviral Drug Umifenovir and Umifenovir Encapsulated in Phospholipids Micelles (Nanosome/Umifenovir) with dsDNA as a Model for Pharmacogenomic Analysis by Electrochemical Methods, Processes, 11(3), 922. DOI:10.3390/pr11030922
Pronina V.V., Kostryukova L.V., Bulko T.V., Shumyantseva V.V. (2023) Interaction of Doxorubicin Embedded into Phospholipid Nanoparticles and Targeted Peptide-Modified Phospholipid Nanoparticles with DNA, Molecules, 28(14), 5317. DOI:10.3390/molecules28145317
Agafonova L., Zhdanov D., Gladilina Yu., Kanashenko S., Shumyantseva V. (2022) A pilot study on an electrochemical approach for assessing transient DNA transfection in eukaryotic cells, Journal of Electroanalytical Chemistry, 920, 116635. DOI:10.1016/j.jelechem.2022.116635
Shumyantseva V.V., Koroleva P.I., Bulko T.V., Sergeev G.V., Usanov S.A. (2022) Predicting drug-drug interactions by electrochemically driven cytochrome P450 3A4 reactions, Drug Metabolism and Personalized Therapy, 37(3), 241-248. DOI:10.1515/dmpt-2021-0116
Agafonova L., Tikhonova E., Sanzhakov M., Kostryukova L., Shumyantseva V. (2022) Electrochemical Studies of the Interaction of Phospholipid Nanoparticles with dsDNA, Processes, 10(11), 2324. DOI:10.3390/pr10112324
Masamrekh R.A., Kuzikov A.V., Filippova T.A., Sherbakov K.A., Veselovsky A.V., Shumyantseva V.V. (2022) Interaction of Abiraterone and Its Pharmacologically Active Metabolite D4A with Cytochrome P450 2C9 (CYP2C9), Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, 16(4), 328-339. DOI:10.1134/S1990750822040059
Shumyantseva V.V., Kuzikov A.V., Masamrekh R.A., Filippova T.A., Koroleva P.I., Agafonova L.E., Bulko T.V., Archakov A.I. (2022) Enzymology on an Electrode and in a Nanopore: Analysis Algorithms, Enzyme Kinetics, and Perspectives, BioNanoScience, 12, 1341-1355. DOI:10.1007/s12668-022-01037-2
Shumyantseva V.V., Bulko T.V., Kuzikov A.V., Masamrekh R.A., Pergushov D.V., Schacher F.H., Sigolaeva L.V. (2020) Electrochemical fingerprint of cytochrome c on a polymer/MWCNT nanocomposite electrode, Mendeleev Communications, 30(3), 299-301. DOI:10.1016/j.mencom.2020.05.012
Shumyantseva V.V., Makhova A.A., Shikh E.V., Bulko T.V., Kuzikov A.V., Masamrekh R.A., Shkel T., Usanov S., Gilep A., Archakov A.I. (2019) Bioelectrochemical Systems as Technologies for Studying Drug Interactions Related to Cytochrome P450, BioNanoScience, 9(1), 79-86. DOI:10.1007/s12668-018-0567-7
Shumyantseva V.V., Bulko T.V., Sigolaeva L.V., Kuzikov A.V., Pogodin P.V., Archakov A.I. (2018) Molecular imprinting coupled with electrochemical analysis for plasma samples classification in acute myocardial infarction diagnostic, Biosensors & Bioelectronics, 99, 216-222. DOI:10.1016/j.bios.2017.07.026
- Archakov A.I., Lisitsa A.V., Ipatova O.M., Misharin A.Yu., Stulov S.V., Shishova D.K., Shumyantseva V.V., Kuzikov A.V., Veselovsky A.V. Derivatives of pregn-17 (20)-ene exhibiting antitumor activity. Patent № 2617698 (RU2015111223A). Registered in the State Register of Inventions of the Russian Federation on April 26th of 2017
- Suprun E.V., Khmeleva S.A., Kiseleva Ya.Yu., Radko S.P., Archakov A.I., Shumyantseva V.V. Electrochemical method for amino acid substitutions and modifications analysis in amyloid-beta peptide. Patent № 2616706 (RU2616706C2). Registered in the State Register of Inventions of the Russian Federation on April 18th of 2017
- Shumyantseva V.V., Bulko T.V., Kuzikov A.V., Suprun E.V., Sigolaeva L.V. Method of express determination of cardiomyoglobin in blood plasma using electrochemical sensor based on carbon nanotubes and molecular imprinted poly-o-phenylenediamine as bioaffinity reagent. Patent № 2633086. Registered in the State Register of Inventions of the Russian Federation on October 11th of 2017
- Suprun E.V., Radko S.P., Mitkevich V.A., Makarov A.A., Archakov A.I., Shumyantseva V.V. Electrochemical method for detecting amino acid substitutions and identifying peptides. Patent № 26330078 (RU2633078C2). Registered in the State Register of Inventions of the Russian Federation on October 11th of 2017
- Suprun E.V., Radko S.P., Khmeleva S.A., Kiseleva Ya.Yu., Archakov A.I., Shumyantseva V.V. Electrochemical method for rapid analysis of complex formation of amyloid-beta with metal ions. Patent №RU2585307. Registered in the State Register of Inventions of the Russian Federation April 1st of 2015
- Shumyantseva V.V., Bulko T.V., Suprun E.V., Kuzikov A.V. Electrochemical biosensor for direct detection of myoglobin based on carbon nanotubes and a molecularly imprinted polymer based on o-phenylenediamine and a method for its preparation. Patent № RU2604688. Registered in the State Register of Inventions of the Russian Federation on November 18th of 2016
- Shumyantseva V.V. Semisynthetic flavocytochrome P450: electron transfer and catalysis. D.Sci.
- Suprun E.V. Electrochemical biosensor systems for analysis of clinically important proteins and peptides. D.Sci.
- Makhova A.A. Regulation of the activity of cytochrome P450 3A4 enzymes by vitamins and vitamin-like substances. D.Sci.
- Rudakov Yu.O. Electrochemical reduction of cytochrome P450 2B4 using nanocomposite electrode materials: stoichiometry and thermodynamics. PhD
- Shumkov A.A. Electrochemical analysis of cardiac markers in blood plasma. PhD
- Kuzikov A.V. Electrochemical monitoring of the catalytic activity of cytochromes P450. PhD
- Valilulina S.G. «Modeling microsomal monooxygenase system using human serum albumin modified with the reactive riboflavin analog 7-α-bromacetyl-10-methylisoalloxazine». Moscow State Academy of Fine Chemical Technology. Department of Chemistry and Technology of Fine Organic Compounds.
- Byakova O.E. «Modeling microsomal monooxygenase system based on cytochrome P450 2B4 containing riboflavin as an electron donor group». Pirogov Russian State Medical University. Faculty of Biomedicine, Department of Biochemistry.
- Avdeenko Yu.L. «Study of the influence of hydrogen peroxide and cytochrome b5 on reactions catalyzed by semisynthetic flavocytochrome 2B4». Pirogov Russian State Medical University. Faculty of Biomedicine, Department of Biochemistry.
- Marsova M.V. «The development of methods for enzyme electrode preparation based on cytochrome P450 2B4». Lomonosov Moscow State University. Faculty of Chemistry, Department of Natural Compound Chemistry.
- Kurnyshova A.V. «The development of method for direct electrochemical immunoanalysis of myoglobin for earlier diagnostics of myocardial infarction». Moscow Engineering Physics Institute. Department №1.
- Kozin M.S. «The development of methods for electrode modification for electroanalysis of nucleotides and oligo(poly)nucleotides». Pirogov Russian National Research Medical University. Faculty of Biomedicine, Department of Biochemistry.
- Masamrekh R.A. «Electroanalysis of cytochrome P450 17A1 as a molecular target for potential antitumor drugs». Pirogov Russian National Research Medical University. Faculty of Biomedicine, Department of Biochemistry.
- Korlenkov A.V. «Electrochemical approach for analysis of drugs». Bachelor’s degree, 2017
- Kuzina E.V. «Electrochemical approach for analysis of ibuprofen». Bachelor’s degree, 2018
- Dementeva A.A. «Determination of diclofenac in biological fluids by electrochemical methods». Bachelor’s degree, 2018
- Antonova A.N. Determination of paracetamol in biological fluids by electrochemical methods». Bachelor’s degree, 2018
- Koroleva P.I. «Electroanalytical properties of cytochrome P450 3A4 and their application for analysis of drug-drug interactions». Master’s degree, 2018
- Saveleva D.V. «Optimization of the electrochemical registration of cytochrome c on electrodes modified with carbon nanotubes». Bachelor’s degree, 2020
- Pronina V.V. «Nanocomposite materials as electrode modifiers for electrochemical analysis of biological systems». Master’s degree, 2022
- Agapitova (Serdyukova) A.S. «Optimization of the parameters for registration of nucleic acids on graphite electrodes». Bachelor’s degree, 2023