Laboratory of Environmental Biotechnology

Konstantin V. Zolotarev

 

 

 

Laboratory of Environmental Biotechnology was founded in 2011. Head of the Laboratory is Marina V. Mikhailova, Ph.D., Ass. Prof.

 

 

RESEARCH

The laboratory specializes in basic and applied research in the following areas:

avatar none zebrafish (Danio rerio) as a model for different studies, i.e.: toxicological (the study of the toxicity of biologically active compounds at the various stages of development); studies in the field of developmental biology (larvae locomotor activity); preparation of biological material for proteomic, genomic, transcriptomic studies;

avatar none fish gerontology studies: a comparative analysis of the chemical composition of the fishes with the various aging rate;

avatar none studying the impact of the extracts of the fish tissues (muscle, roe, ovarian liquid) on the cell culture senescence and its recovery after the oxidative stress;

avatar none development of the food supplies based on the fish tissue extracts with beneficial effects on overall life quality including healthy lifespan;

avatar none studying chemical composition of sturgeon ovarian fluid and other fish processing waste; discovery of bioactive substances and assessment of their nutraceutical and treatment-and-prophylactic potential;

avatar none measurement of the content of metals (including essential nutrients and toxic heavy metals) in the samples of various nature (water, biological tissues, soil, sediments, food, feed, etc.).

RESULTS

  • The toxic action of some substances (doxorubicin as a free substance and in phospholipid micelles, Co2+, Cd2+, Ni2+, Cu2+, Pb2+, Hg2+, CdSe/CdS/ZnS-based quantum dots, nanoparticles of diamond, Si, SiO2, TiO2, hydroxyapatite) at a Danio rerio model at the various stages of development has been studied.
  • The mechanism of Cd2+ and Cu2+ toxic action has been studied. The changes of proteomic profiles of embryos and adult Danio rerio organs induced by these ions have been figured out. Cd2+ induces the reduction of Ca2+-binding protein content, while Cu2+ induces changes of the antioxidant system enzymes and mitochondrial enzymes content.
  • It has been found that in the Cd-accumulated nervous tissue of Cd2+-exposed carp the Ca2+-transporting membrane channel subunit (NR2A) gene expression is upregulated. It confirms the previously hypothesized statement that a heavy metal cation with a similar to Ca2+ ionic radius can irreversibly block the calcium channels.
  • The correlations between acute toxicity endpoints of different substances for different test organisms (rodents, adult fish, fish embryos) and a chemical parameter of the substances (logP) have been figured out.
  • A method for mathematical modeling of simultaneous coagulation and sedimentation of the suspended particles has been developed.
  • The proteomic and metabolomic profiles of the fish with various aging speeds have been compared. The differences in the content of proteins and low-molecular compounds associated with aging and metabolism have been figured out.
  • It's been found that biologically active substances of the lyophilized sturgeon ovarian fluid and of the muscle extracts of pike, sterlet, pink salmon and, to a lesser degree, biologically active substances of the muscle extracts of perch and Russian sturgeon and of the perch roe extract are capable of accelerating the fibroblast culture recovery after H2O2-induced oxidative stress. The lyophilized sturgeon ovarian fluid induces the reduction of the percentage of the SA-β-Gal-positive cells without proliferation growth which shows that the state of the cells has improved. A technology of a pike muscle extract-based food supply manufacturing has been developed (Patent RU 2657511), the food supply has been registered by Rospotrebnadzor in Russia (Registration certificate no. 77.99.11.003.R.001257.04.21 and 77.99.11.003.R.001258.04.21).
  • High antioxidant activity of the lyophilized sturgeon ovarian fluid has been found using a coulometric method.
  • Peptidomic profiling of the fluid resulted in finding a peptide with a potentially high antimicrobial activity, according to mathematical modeling results. Antimicrobial activity has been confirmed for several strains of opportunistic pathogens.
  • A large set of data of ecological monitoring of the water bodies including heavy metals content in water, water sediments and fish tissues (Registered Databases no. 2015621006 and 2016620137) and fish parasitological examination data (Registered Databases no. 2017620551 and 2021620008) has been gathered.
  • An algorithm for analyzing the distribution of heavy metals between fish tissues has been developed. The algorithm has been successfully tested using the gathered data of metals content in the pike tissues.

avatar none  Mikhailova M., Zolotarev K., Mikhailov A., Lapin A., Nakhod V. (2023) Antioxidant activity of freeze-dried Siberian sturgeon (Acipenser baerii) ovarian fluid, E3S Web of Conferences, 381, 01075. DOI:10.1051/e3sconf/202338101075

avatar none  Mikhaylova M.V., Zolotarev K.V., Bodoev N.V., Mikhaylov A.N., Nakhod V.I., Lapin A.A., Zelenkov V.N., Samarsky V.G. (2022) Total antioxidant activity of extracts of tissues of Salmonidae, Percidae and Acipenseridae families fish, Butlerov Communications, 69(3), 131-138. DOI:10.37952/ROI-jbc-01/22-69-3-131

avatar none  Mikhailova M., Zolotarev K., Nakhod V., Farafonova T., Mikhailov A. (2022) Nutritional value of black solder fly (Hermetia illucens) eggs and larvae reared on fermented milk industry waste as an ingredient of fish feed, E3S Web of Conferences, 363, 03017. DOI:10.1051/e3sconf/202236303017

avatar none  Lapin A.A., Zelenkov V.N., Zolotarev K.V., Mikhailov A.N., Bodoev N.V., Mikhailova M.V. (2021) Total antioxidant activity of extraction products of muscles and roe of northern pike (Esox lucius), Butlerov Communications, 65(1), 114-119. DOI:10.37952/ROI-jbc-01/21-65-1-114

avatar none  Zolotarev K.V., Mikhailov A.N., Mikhailova M.V., Nakhod K.V., Nakhod V.I., Bodoev N.V., Zhdanov D.D. (2021) Tissue-Specific Expression of Genes Involved in Cellular Transportation in Common Carp (Cyprinus carpio) Exposed to Cadmium, Bulletin of Environmental Contamination and Toxicology, 106, 978-982. DOI:10.1007/s00128-021-03270-z

avatar none  Zarubin N.Yu., Kharenko E.N., Bredikhina O.V., Arkhipov L.O., Zolotarev K.V., Mikhailov A.N., Nakhod V.I., Mikhailova M.V. (2021) Application of the Gadidae Fish Processing Waste for Food Grade Gelatin Production, Marine Drugs, 19(8), 455. DOI:10.3390/md19080455

avatar none  Novikov V.Y., Shumskaya N.V., Mukhin V.A., Zolotarev K.V., Mikhailov A.N., Nakhod V.I., Mikhailova M.V. (2021) Chemical Characterization of Atlantic Cod (Gadus morhua) Collagen Hydrolyzed Using Enzyme Preparation Derived from Red King Crab (Paralithodes camtschaticus) and Its Potential as a Core Component of Bacterial Culture Medium, Marine Drugs, 19(8), 472. DOI:10.3390/md19080472

avatar none  Mikhailova M.V., Prozorovskiy V.N., Zolotarev K.V., Ipatova O.M., Mikhailov A.N., Kharenko E.N., Artemov A.V. (2020) Carnosine Levels in the Muscle Tissues of Sturgeons and Their Hybrids, Applied Biochemistry and Microbiology, 56(3), 352-355. DOI:10.1134/S0003683820030084

avatar none  Maslov D.L., Trifonova O.P., Mikhailov A.N., Zolotarev K.V., Nakhod K.V., Nakhod V.I., Belyaeva N.F., Mikhailova M.V., Lokhov P.G., Archakov A.I. (2019) Comparative Analysis of Skeletal Muscle Metabolites of Fish with Various Rates of Aging, Fishes, 4(2), 25. DOI:10.3390/fishes4020025

avatar none  Mikhailova M.V., Belyaeva N.F., Kozlova N.I., Zolotarev K.V., Mikhailov A.N., Podushka S.B. (2018) The Sturgeon Ovarian Liquid and the Perch Roe Extract are Able to Enhance the Recovery of the Fibroblasts after their Stress-induced Premature Senescence, Biomedical Chemistry: Research and Methods, 1(2), e00011. DOI:10.18097/BMCRM00011

avatar none  Zolotaryov K., Mikhailova M., Nakhod K., Mikhailov A. (2018) Algorithm to analyse distribution of heavy metals in fish tissues on the example of northern pike, Principles of the Ecology, 7(3), 25-37. DOI:10.15393/j1.art.2018.7742

avatar none  Trifonova O.P., Maslov D.L., Mikhailov A.N., Zolotarev K.V., Nakhod K.V., Nakhod V.I., Belyaeva N.F., Mikhailova M.V., Lokhov P.G., Archakov A.I. (2018) Comparative Analysis of the Blood Plasma Metabolome of Negligible, Gradual and Rapidly Ageing Fishes, Fishes, 3(4), 46. DOI:10.3390/fishes3040046

avatar none  Zolotarev K.V., Belyaeva N.F., Mikhailov A.N., Mikhailova M.V. (2017) Dependence between LD50 for Rodents and LC50 for Adult Fish and Fish Embryos, Bulletin of Experimental Biology and Medicine, 162(10), 445-450. DOI:10.1007/s10517-017-3636-y

avatar none  Mikhailova M.V., Belyaeva N.F., Kozlova N.I., Zolotarev K.V., Mikhailov A.N., Berman A.E., Archakov A.I. (2017) Protective action of fish muscle extracts against cellular senescence induced by oxidative stress, Biomeditsinskaya Khimiya, 63(4), 351-355. DOI:10.18097/PBMC20176304351

avatar none  Belyaeva N.F., Zolotarev K.V., Kashirtseva V.N., Druzhilovskaya O.S., Ignatov D.V., Ipatova O.M., Mikhailova M.V. (2014) Toxicity evaluation of gold and diamond nanoparticles, ScienceRise, 5№4(5), 30-34. DOI:10.15587/2313-8416.2014.34227

  • IBMC-EcoBioTech LLC;
  • Mustela-AGRO LLC (Skolkovo Innovation Center resident);
  • Moscow Department for Environmental Management and Protection;
  • Mosecomonitoring;
  • Federal Agency for Fisheries of Russia;
  • Russian Federal Research Institute of Fisheries and Oceanography (VNIRO);
  • Astrakhan State Technical University
  • Development and testing of methods of studying toxic action of heavy metals based on laboratory modeling and fish tissue chemical analysis (completed and approved for defense, Ph.D.)
  • Identifying the toxic substances in organs and tissues of the most widespread fish species of the Central region of Russia (graduate)
  • Studying the bioaccumulation of heavy metals in aquatic organisms, the northern pike is a sample (graduate)
  • The ecological estimation of the state of the Uglich and Ivankovo water reservoirs using toxic substances bioaccumulation levels in aquatic organisms (graduate)