Laboratory of Mass Spectrometric Metabolomic Diagnostics
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RESEARCH
The main work of the laboratory is related to the metabolomics studies of body fluids for characterizing of the functioning of a biological object in a pathological state and the development of diagnostic methods based on metabolites – low-molecular (up to 1500 Da) organic compounds that are substrates, intermediates or products of biochemical reactions. Metabolomics is the final of the post-genomics disciplines, and the identified low-molecular substances provide comprehensive information about the patient's condition, the presence of pathological processes, the effectiveness of drug therapy, the risk of diseases initiation, and the effects of environmental factors on the body. The main research material is human blood plasma/serum as the most informative biological fluid available in the clinic for laboratory diagnostics. The main research method for metabolites analysis in the blood plasma is a mass spectrometry analysis.
PROJECTS
- Metabolomics profiling of blood plasma of patients with socially significant diseases
Mass spectrometry analysis of blood plasma/serum metabolites for the development of methods for diagnosing and assessing the risk of initiation of socially significant diseases – oncology (prostate cancer, lung cancer, colorectal cancer, kidney cancer, etc.), diseases of the endocrine system (obesity, impaired glucose tolerance, diabetes), neurodegenerative and cardiovascular diseases.
Maslov D.L., Trifonova O.P., Lichtenberg S., Balashova E.E., Mamedli Z.Z., Alferov A.A., Stilidi I.S., Lokhov P.G., Kushlinskii N.E., Archakov A.I. (2023) Blood Plasma Metabolome Profiling at Different Stages of Renal Cell Carcinoma, Cancers, 15(1), 140. DOI:10.3390/cancers15010140
Trifonova O.P., Maslov D.L., Balashova E.E., Lokhov P.G. (2023) Current State and Future Perspectives on Personalized Metabolomics, Metabolites, 13(1), 67. DOI:10.3390/metabo13010067
Lokhov P.G., Balashova E.E., Trifonova O.P., Maslov D.L., Grigoriev A.I., Ponomarenko E.A., Archakov A.I. (2023) Mass Spectrometric Blood Metabogram: Acquisition, Characterization, and Prospects for Application, International Journal of Molecular Sciences, 24(2), 1736. DOI:10.3390/ijms24021736
Lokhov P.G., Balashova E.E., Trifonova O.P., Maslov D.L., Plotnikova O.A., Sharafetdinov K.K., Nikityuk D.B., Tutelyan V.A., Ponomarenko E.A., Archakov A.I. (2023) Clinical Blood Metabogram: Application to Overweight and Obese Patients, Metabolites, 13(7), 798. DOI:10.3390/metabo13070798
Balashova E.E., Maslov D.L., Trifonova O.P., Lokhov P.G., Archakov A.I. (2022) Metabolome Profiling in Aging Studies, Biology, 11(11), 1570. DOI:10.3390/biology11111570
Trifonova O.P., Maslov D.L., Balashova E.E., Lichtenberg S., Lokhov P.G. (2022) Potential Plasma Metabolite Biomarkers of Diabetic Nephropathy: Untargeted Metabolomics Study, Journal of Personalized Medicine, 12(11), 1889. DOI:10.3390/jpm12111889
Lokhov P.G., Maslov D.L., Lichtenberg S., Trifonova O.P., Balashova E.E. (2021) Holistic Metabolomic Laboratory-Developed Test (LDT): Development and Use for the Diagnosis of Early-Stage Parkinson’s Disease, Metabolites, 11(1), 14. DOI:10.3390/metabo11010014
Trifonova O.P., Maslov D.L., Balashova E.E., Lokhov P.G. (2021) Mass spectrometry-based metabolomics diagnostics - myth or reality?, Expert Review of Proteomics, 18(1), 7-12. DOI:10.1080/14789450.2021.1893695
Lichtenberg S., Trifonova O.P., Maslov D.L., Balashova E.E., Lokhov P.G. (2021) Metabolomic Laboratory-Developed Tests: Current Status and Perspectives, Metabolites, 11(7), 423. DOI:10.3390/metabo11070423
Lokhov P.G., Trifonova O.P., Maslov D.L., Balashova E.E. (2020) In Situ Mass Spectrometry Diagnostics of Impaired Glucose Tolerance Using Label-Free Metabolomic Signature, Diagnostics, 10(12), 1052. DOI:10.3390/diagnostics10121052