Laboratory of Medical Biotechnology

  Blinova V.G., Gladilina Y.A., Abramova A.A., Eliseeva D.D., Vtorushina V.V., Shishparenok A.N., Zhdanov D.D. (2024) Modulation of Suppressive Activity and Proliferation of Human Regulatory T Cells by Splice-Switching Oligonucleotides Targeting FoxP3 Pre-mRNA, Cells, 13(1), 77. DOI:10.3390/cells13010077

  Latysheva A.S., Zolottsev V.A., Veselovsky A.V., Scherbakov K.A., Morozevich G.E., Zhdanov D.D., Novikov R.A., Misharin A.Y. (2023) Oxazolinyl derivatives of androst-16-ene as inhibitors of CYP17A1 activity and prostate carcinoma cells proliferation: Effects of substituents in oxazolinyl moiety, Journal of Steroid Biochemistry and Molecular Biology, 230, 106280. DOI:10.1016/j.jsbmb.2023.106280

  Dobryakova N.V., Zhdanov D.D., Sokolov N.N., Aleksandrova S.S., Pokrovskaya M.V., Kudryashova E.V. (2023) Rhodospirillum rubrum L-Asparaginase Conjugates with Polyamines of Improved Biocatalytic Properties as a New Promising Drug for the Treatment of Leukemia, Applied Sciences (Switzerland), 13(5), 3373. DOI:10.3390/app13053373

  Shishparenok A.N., Furman V.V., Zhdanov D.D. (2023) DNA-Based Nanomaterials as Drug Delivery Platforms for Increasing the Effect of Drugs in Tumors, Cancers, 15(7), 2151. DOI:10.3390/cancers15072151

  Dobryakova N., Zhdanov D., Dumina M., Aleksandrova S., Pokrovskaya M., Genin A., Shishparenok A., Zhgun A., Kudryashova E.V. (2023) Thermal Inactivation Mechanism and Structural Features Providing Enhanced Thermal Stability of Hyperthermophilic Thermococcus sibiricus L-Asparaginase in Comparison with Mesophilic and Thermophilic L-Asparaginases, Catalysts, 13(5), 832. DOI:10.3390/catal13050832

  Blinova V.G., Vasilyev V.I., Rodionova E.B., Zhdanov D.D. (2023) The Role of Regulatory T Cells in the Onset and Progression of Primary Sjögren’s Syndrome, Cells, 12(10), 1359. DOI:10.3390/cells12101359

  Dumina M., Zhdanov D., Zhgun A., Pokrovskaya M., Aleksandrova S., Veselovsky A., El’darov M. (2023) Enhancing the Catalytic Activity of Thermo-Asparaginase from Thermococcus sibiricus by a Double Mesophilic-like Mutation in the Substrate-Binding Region, International Journal of Molecular Sciences, 24(11), 9632. DOI:10.3390/ijms24119632

  Shishparenok A.N., Gladilina Y.A., Zhdanov D.D. (2023) Engineering and Expression Strategies for Optimization of L-Asparaginase Development and Production, International Journal of Molecular Sciences, 24(20), 15220. DOI:10.3390/ijms242015220

  Dobryakova N.V., Zhdanov D.D., Sokolov N.N., Aleksandrova S.S., Pokrovskaya M.V., Kudryashova E.V. (2022) Improvement of Biocatalytic Properties and Cytotoxic Activity of L-Asparaginase from Rhodospirillum rubrum by Conjugation with Chitosan-Based Cationic Polyelectrolytes, Pharmaceuticals, 15(4), 406. DOI:10.3390/ph15040406

  Shumyantseva V., Bulko T., Pronina V., Kanashenko S., Pokrovskaya M., Aleksandrova S., Zhdanov D. (2022) Electroenzymatic model system for the determination of catalytic activity of Erwinia carotovora L-asparaginase, Processes, 10(7), 1313. DOI:10.3390/pr10071313

  Agafonova L., Zhdanov D., Gladilina Yu., Kanashenko S., Shumyantseva V. (2022) A pilot study on an electrochemical approach for assessing transient DNA transfection in eukaryotic cells, Journal of Electroanalytical Chemistry, 920, 116635. DOI:10.1016/j.jelechem.2022.116635

  Tawfik H.O., El-Hamaky A.A., El-Bastawissy E.A., Shcherbakov K.A., Veselovsky A.V., Gladilina Y.A., Zhdanov D.D., El-Hamamsy M.H. (2022) New Genetic Bomb Trigger: Design, Synthesis, Molecular Dynamics Simulation, and Biological Evaluation of Novel BIBR1532-Related Analogs Targeting Telomerase against Non-Small Cell Lung Cancer, Pharmaceuticals, 15(4), 481. DOI:10.3390/ph15040481

  Pokrovskaya M.V., Pokrovsky V.S., Aleksandrova S.S., Sokolov N.N., Zhdanov D.D. (2022) Molecular Analysis of L-Asparaginases for Clarification of the Mechanism of Action and Optimization of Pharmacological Functions, Pharmaceutics, 14(3), 559. DOI:10.3390/pharmaceutics14030599

  Neborak E.V., Kaldybayeva A.B., Bey L., Malmakova A.Y., Tveritinova A.S., Hilal A., Yu V.K., Ploskonos M.V., Komarova M.V., Agostinelli E., Zhdanov D.D. (2022) Anticancer Cytotoxic Activity of Bispidine Derivatives Associated with the Increasing Catabolism of Polyamines, Molecules, 27(12), 3872. DOI:10.3390/molecules27123872

• Modified TsA gene encoding thermostable L-asparaginase from the archaea Thermococcus sibiricus, recombinant strain of Escherichia coli - producer of thermostable L-asparaginase and a method for purifying this enzyme, 2022, 2022106430/10(013385) Dumina M.V., Zhgun A.A. , Aleksandrova S.S., Pokrovskaya M.V., Sokolov N.N. Zhdanov D.D.
• A method of producing a recombinant polypeptide TnaCheY-mut, recombinant polypeptide TnaCheY-mut, recombinant plasmid pGD TnaCheY-mut gene encoding TnaCheY-mut, strain Escherichia coli - produtsent recombinant polypeptide TnaCheY - mut, 2018 2018143308, Grishin D.V., Sokolov N.N., Pokrovsky M.V., Alexandrov S.S., Pokrovsky V.S.
• The method of obtaining the recombinant elastomeric domain Trib-2 mut, the genetic engineering construct pGD Trib 2 mut, which determines the biosynthesis of Trib-2 mut in E cells. coli, strain-producing E. coli M15/pGD Trib 2 mut and a method for producing a polymer material based on this protein, 2015, 2015139343, Grishin D.V., Sokolov N.N., Abakumova O.Yu., Podobed O.V., Pokrovskaya M.V., Alexandrov S.S.
• A method of producing a substance stimulating regeneration of damaged liver, 2014, 2,548,750, Halperin E.I., Dyuzheva T.G., Abakumova O.Yu., Platonova L.V.
• The recombinant plasmid DNA pACYC_LANS (KM, the strain Escherichia coli BL21 (DE3, transformed with a recombinant DNA pACYC_LANS (KM, and a method for producing recombinant L-asparaginase Erwinia carotovora, 2010, 2,441,916, Sidoruk K.V., Bogusz V.G., Eldar M.A., Goncharova O.V., Chugunova N.M., Pokrovsky M.V., Alexandrov S.S., Omelyanyuk N.M., Sokolov N.N.
• A method of obtaining a substance of recombinant L-asparaginase Erwinia carotovora, 2010, Karasev V.S., Bochkova O.P., Chugunov A.M., Melik-Nubarov N.S., Grozdova I.D., Chernovskaya T.V., Denisov L.A., Rudenko E.G., Morozova E.L., Bogush V.G., Sidoruk K.V., Koltun I.O., Skatova G.E., Abakumova O.Yu., Podobed O.V., Sokolov N.N.
• A method for detecting strains of bacteria producing L-asparaginase, 2008, 2398876, Pokrovsky M.V., Sokolov N.N., Pokrovsky V.S., Archakov A.I., Gusev D.A.
• Means of exhibiting anti-tumor activity, in 2008, 2,376,020, Tochilkin A.I., Belyaeva N.F., Abakumov O.Yu., Podobed O.V.
• PCT - patent, 2003, WO03018742, Sokolov N.N. et al.
• The recombinant plasmid DNA pACYCLANS for transfer and expression in cells of Escherichia coli gene L-asparaginase Ervinia Carotovora (ECAR-LANS) and method for its preparation by recombinant ECAR-LANS of biomass of strain E.coli-producer, 2002, 2,224,797, Eldar M.A., Zhgun A.A., Gervaziev Yu.In., Aleksandrova S.S., Bogusz V.R., Sidoruk K.V., Sveshnikov E.V., Borisov A.A., Omel'yanyuk N.M., Archakov A.I., Scriabin K.R., Sokolov N.N.
• Gene L-asparaginase Ervinia Carotovora and strain Escherichia coli VKPM №In-8174 - producing L-asparaginase Ervinia Carotovora, 2001, 2,221,868, Eldar M.A., Zhgun A.A. , Gervaziev Yu.V., Aleksandrova S.S., Omel'yanyuk N.M ., Archakov A.I., Scriabin K.R ., Sokolov N.N
• A method for the photodynamic treatment of acute and chronic purulent sinusitis, 2002 , 2228775, Nasedkin A.N., Reshetnikov A.V., Grachev S.V., Zalevsky I.L., Zenger V.G., Kemov Yu.V., Selin V.N., Abakumov O.Yu. and others
• The photosensitizer and a method for its preparation, 2001, 1,283,956, Resetnicov A.V., Zalewski I.L., Kemov Y.V., Ivanov A., Karmenyan A.A., Gradyushko A.T., Laptev V.P., Neugodova N.P., Abakumov O.Yu. and etc.
• A method for producing restriction endonuclease BspDI, 1990, 1,707,077, Sokolov N.N., Anikeycheva N.V., Fmtsner A.B., Samko O.T. Kalugin A.A., Horoshutina E.B., Librik G.I.
• Bacterial strain of Bacillus brevis - producing restriction BBV of Bi, 1990 1,832,129, Sokolov N.N., Anikeycheva N.V., Eldar M.A., Fintser A.B., Karpychev I.V., Kalugin A.A., Samko O.T., Horoshutina E.B., Librik G.I.
• Bacterial strain of Bacillus alvei - producing restriction Bav BII, 1990 1,761,803, Sokolov N.N., Fodor I.I., Fintser A.B.
• The strain of the bacterium Bacillus coagulans - producer of restriction enzyme Bco AI, 1990, 1761804, Sokolov N.N., Anikeicheva N.V., Eldarov M.A., Fintzer A.B., Karpychev I.V., Kalugin A.A., Samko O.T., Horoshutina E.B., Librik G.I.
• Bacterial strain of Bacillus alvei - producing restriction Bav AI, 1990, 1,678,833, Sokolov N.N, Fodor I.I.
• A method for producing restriction endonuclease BbvAI, 1990, 1,712,415, Sokolov N.N., Fitsner A.B., Anikeycheva N.V., Horoshutina E.B., Samko O.T., Kalugin A.A.
• The strain of bacteria of Achromobacter an agile - producing restriction Aag525I, 1989, 1,648,976 Sokolov N.N.
• Bacterial strain of Lactobacillus plantarum - producing restriction LPlI, 1989, 1,678,832, Sokolov N.N., Fodor I.I,. Fintser A.B.,. Anikeycheva N.V., Kalugin A.A., Horoshutina E.B., Samko O.T., Kovalenko N.A., Eldar M.A.
• The nutrient medium for culturing bacteria of the strain Pseudomonas airuginosa GISCO 134-producing restriction enzyme Rye II-, 1988, 1,561,506, Boykov Y.N., Viestur Z.A., Sokolov N.N., Fintser A.B., Anikeycheva N.V.
• A process for preparing the solid phase support with a trityl groups, 1988, 1,651,262, Cherkasov T.A., Vonsky V.G ., Leikin Y.A., Kovalenko N.A., Sokolov N.N.
• A method for producing restriction endonucleases PaeI, 1988, 1,592,333, Sokolov N.N., Fitsner A.B., Boykov Y.N., Horoshutina E.B., Kalugin A.A.
• A method of producing a substance, which stimulates the proliferative process card endocrine pancreas, 1986, 1,412,061, Halperin E.I., Abakumova O.Yu., Ionochkina N.N.
• A strain of Pseudomonas Aeruginosa-18 - producer endonuclease restriction Rae 11, 1985, 1,314,669, Sokolov N.N., Fodor I.I, Fintser A.B., Horoshutina E.B., Anikeycheva N.V., Samko O.T., Kolosha V.O.
• A method for producing restriction endonuclease recognizes and cleaves the nucleotide sequence 5'ACATC↓C, 1249935, 1984, Sokolov N.N., Fodor I.I., Fitsner A.B., Horoshutina È.B,, Anikeycheva N.V., Kolosha V.O., Vautrin I.I.
• A method for determining the activity of restriction endonucleases, 1983, 1,170,777, Heyslere M.Ya., Zilbere A.M., Sokolov N.N., Votrin I.I., Fintser A.B., Filipchenkova L.P., Shiverskih M.M.
• A method for producing an enzyme preparation with a restriction enzyme activity, 1983, 1,160,743, Heyslere M.Ya., Shiverskih M.W., Ansberg S.E., Yasukevich A.K., Sproģe V.A., Fitsner A.B., Sokolov N.N., Votrin I.I.
• A method for producing restriction enzymes, 1983, 1,163,637, Heyslere M.Ya., Shiverskih M.W., Ansberg S.E., Fitsner A.B., Sokolov N.N., Votrin I.I.
• A method of producing a restriction enzyme Bam HI, 1982, 1,078,930, Heyslere M.YA., Filipchenkova LP, Šmite I.A., Ansberg S.E., Fintser A.B., Sokolov N.N., Votrin I.I.
• The nutrient medium for the cultivation of Bacillus amyloliquefaciens - producer of BamH I restrictase, 1982, 1095642, Šmite I.A., Viesture Z.A., Heyslere M.Ya., Ansberg S.E., Aren A.K, Votrin I.I., Sokolov N.N.

• Center for Biotechnology RAS, group of genetic engineering of mushrooms, laboratory of biomedical research
• Federal State Institution Scientific Research Center of Russia (Serpukhov, Moscow Region)
• Biotechnological company "CJSC Biocad" (Petrovo-Dalnee, Moscow Region)
• Russian Center for oncology research
• "BioChemMak" ST LLC
• SSC RF Federal State Unitary Enterprise "GosNIIgenetika"