Laboratory of Environmental Biotechnology

CONTACTS

  +7-499-246-36-31

  +7-499-130-06-64

Website

Nikolay V. Bodoev D.SC., professor

Anna I. Telkova

Yury A. Kozlov

RESEARCH

Laboratory of Environmental Biotechnology was founded in 2011. Head of the Laboratory Marina V. Mikhailova, Ph.D., Ass. Prof., a full member of the International Academy of Sciences of Ecology, Safety of Man and Nature (MANEB). The laboratory specializes in basic and applied research in the following areas:

- Zebrafish (Danio rerio) as a model for different studies, i.e.: toxicological (the study of the toxicity of biologically active compounds at the various stages of development); studies in the field of developmental biology (larvae locomotor activity); preparation of biological material for proteomic, genomic, transcriptomic studies;

- Fish gerontology studies: a comparative analysis of the chemical composition of the fishes with the various aging rate;

- Studying the impact of the extracts of the fish tissues (muscle, roe, ovarian liquid) on the cell culture senescence and its recovery after the oxidative stress;

- Development of the food supplies based on the fish tissue extracts with beneficial effects on overall life quality including healthy lifespan;

- Designing the formulations and producing the prototypes of fish feeds containing innovative components for different development stages of valuable fish species;

- Biological and fish farming research of water bodies (including the ones located in the specially protected areas of nature in Moscow) and designing the programs for their recovery and application for fish breeding;

- Measurement of the content of metals (including essential nutrients and toxic heavy metals) in the samples of various nature (water, biological tissues, soil, sediments, food, feed, etc.).

Partners:

  • IBMC-EcoBioTech LLC;
  • Mustela-AGRO LLC (Skolkovo Innovation Center resident);
  • Moscow Department for Environmental Management and Protection;
  • Mosecomonitoring;
  • Federal Agency for Fisheries of Russia;
  • Russian Federal Research Institute of Fisheries and Oceanography (VNIRO);
  • Astrakhan State Technical University

Education:

  • The ecological and toxicological features of the impact of heavy metals on fish embryogenesis and development (is currently being performed, Ph.D.)
  • Identifying the toxic substances in organs and tissues of the most widespread fish species of the Central region of Russia (graduate)
  • Studying the bioaccumulation of heavy metals in aquatic organisms, the northern pike is a sample (graduate)
  • The ecological estimation of the state of the Uglich and Ivankovo water reservoirs using toxic substances bioaccumulation levels in aquatic organisms (graduate)

RESULTS

  • The toxic action of some substances (doxorubicin as a free substance and in phospholipid micelles, Co2+, Cd2+, Ni2+, Cu2+, Pb2+, Hg2+, CdSe/CdS/ZnS-based quantum dots, nanoparticles of diamond, Si, SiO2, TiO2, hydroxyapatite) at a Danio rerio model at the various stages of development has been studied.

  • The mechanism of Cd2+ and Cu2+ toxic action has been studied. The changes of proteomic profiles of embryos and adult Danio rerio organs induced by these ions have been figured out. Cd2+ induces the reduction of Ca2+-binding protein content, while Cu2+ induces changes of the antioxidant system enzymes and mitochondrial enzymes content.

  • It has been found that in the Cd-accumulated nervous tissue of Cd2+-exposed carp the Ca2+-transporting membrane channel subunit (NR2A) gene expression is upregulated. It confirms the previously hypothesized statement that a heavy metal cation with a similar to Ca2+ ionic radius can irreversibly block the calcium channels.

  • The correlations between acute toxicity endpoints of different substances for different test organisms (rodents, adult fish, fish embryos) and a chemical parameter of the substances (logP) have been figured out.

  • A method for mathematical modeling of simultaneous coagulation and sedimentation of the suspended particles has been developed.

  • The proteomic and metabolomic profiles of the fish with various aging speeds have been compared. The differences in the content of proteins and low-molecular compounds associated with aging and metabolism have been figured out.

  • It's been found that biologically active substances of the lyophilized sturgeon ovarian liquid and of the muscle extracts of pike, sterlet, pink salmon and, to a lesser degree, biologically active substances of the muscle extracts of perch and Russian sturgeon and of the perch roe extract are capable of accelerating the fibroblast culture recovery after H2O2-induced oxidative stress. The lyophilized sturgeon ovarian liquid induces the reduction of the percentage of the SA-β-Gal-positive cells without proliferation growth which shows that the state of the cells has improved. A technology of a pike muscle extract-based food supply manufacturing has been developed (Patent RU 2657511), the food supply is currently being registered by Rospotrebnadzor in Russia.

  • High antioxidant activity of the lyophilized sturgeon ovarian liquid has been found using a coulometric method. Peptidomic profiling of the liquid resulted in finding a peptide with a potentially high antimicrobial activity, according to mathematical modeling results

  • The fish feeds with innovative components (fur farming wastes) for juvenile and adult trout, sterlet and sturgeon have been developed and successfully tested (Patent RU 2708923). The feeds are demanded well by the market and at the same time, their products can help to solve an ecological problem of waste utilization.

  • A large set of data of ecological monitoring of the water bodies including heavy metals content in water, water sediments and fish tissues (Registered Databases no. 2015621006 and 2016620137) and fish parasitological examination data (Registered Database no. 2017620551) has been gathered. The biological and fish farming researches of water bodies (including the ones located in the specially protected areas of nature in Moscow) have been carried out.

  • An algorithm for analyzing the distribution of heavy metals between fish tissues has been developed. The algorithm has been successfully tested using the gathered data of metals content in the pike tissues.